Category Archives: Education

Measuring Protein Bond Strength with Optical Tweezers

Using a light touch to measure protein bonds

MIT researchers have developed a novel technique to measure the strength of the bonds between two protein molecules important in cell machinery: Gently tugging them apart with light beams. “It’s really giving us a molecular-level picture of what’s going on,” said Matthew Lang, an assistant professor of biological and mechanical engineering

The researchers studied the interactions between the proteins by pinning one actin filament to a surface and controlling the motion of the second one with a beam of light. As the researchers tug on a bead attached to the second filament, the bond mediated by the actin-binding protein eventually breaks.

With this technique, the researchers can get a precise measurement of the force holding the proteins together, which is on the order of piconewtons (10-12 newtons).

Related: Neuroengineers Use Light to Silence Overactive NeuronsSlowing Down LightFoldit, the Protein Folding Game

Germany Looking to Kindergarten for Engineering Future

German groups seek next crop of engineers in the kindergarten

Germany’s shortage of engineers has become so acute that some of its leading companies are turning to kindergartens to guarantee future supplies.

Groups such as Siemens and Bosch are among hundreds of companies giving materials and money to kindergartens to try to interest children as young as three in technology and science.

Many European countries from Switzerland to Spain suffer shortages of graduates. But the problem is especially acute in Germany, renowned as a land of engineering. German companies have 95,000 vacancies for engineers and only about 40,000 are trained, according to the engineers’ association.

“It is a new development in that we have seen we need to start very early with children. Starting at school is not good enough – we need to help them to understand as early as possible how things work,” said Maria Schumm-Tschauder, head of Siemens’ Generation21 education programme.

Siemens has provided about 3,000 “discovery boxes” filled with science experiments for three- to six-year-olds to kindergartens throughout Germany, at a cost to the company of €500 (£395) a box. It also trains kindergarten teachers on how to use them as well as providing similar boxes around the world to pre-schools from China and South Africa to Ireland and Colombia.

Related: Fun k-12 Science and Engineering LearningMiddle School EngineersSarah, aged 3, Learns About SoapLego LearningRanking Universities WorldwideScience Toys You Can Make With Your Kids

Protecting the Food Supply

A few weeks ago we posted about Tracking Down Tomato Troubles as another example of the challenges of scientific inquiry. Too often, in the rare instances that science is even discussed in the news, the presentation provides the illusion of simple obvious answers. Instead it is often a very confusing path until the answers are finally found (posts on scientific investigations in action). At which time it often seems obvious what was going on. But to get to the solutions we need dedicated and talented scientists to search for answers.

Now the CDC is saying tomatoes might not be the source of the salmonella after all: CDC investigates possible non-tomato salmonella sources.

Federal investigators retraced their steps Monday as suspicions mount that fresh unprocessed tomatoes aren’t necessarily causing the salmonella outbreak that has sickened hundreds across the USA.

Three weeks after the Food and Drug Administration warned consumers to avoid certain types of tomatoes linked to the salmonella outbreak, people are still falling ill, says Robert Tauxe with the Centers for Disease Control and Prevention. The latest numbers as of Monday afternoon were 851 cases, some of whom fell ill as recently as June 20, says Tauxe, deputy director of the CDC’s division of foodborne diseases.

The CDC launched a new round of interviews over the weekend. “We’re broadening the investigation to be sure it encompasses food items that are commonly consumed with tomatoes,” Tauxe says. If another food is found to be the culprit after tomatoes were recalled nationwide and the produce industry sustained losses of hundreds of millions of dollars, food safety experts say the public’s trust in the government’s ability to track foodborne illnesses will be shattered.

“It’s going to fundamentally rewrite how we do outbreak investigations in this country,” says Michael Osterholm of the Center for Infectious Disease Research and Policy at the University of Minnesota. “We can’t let this investigation, however it might turn out, end with just the answer of ‘What caused it?’ We need to take a very in-depth look at foodborne disease investigation as we do it today.”

I am inclined to believe the FDA is not enough focused on food safety. Perhaps we are not funding it enough, but we sure are spending tons of money on something so I can’t believe more money needs to be spent. Maybe just fewer bills passed (that the politicians don’t even bother to read) with favors to special interests instead of funding to support science and food safety. Or perhaps we are funding enough (though I am skeptical of this contention) and we just are not allowing food safety to get in the way of what special interests want (so we fund plenty for FDA to have managed this much better, to have systems in place that would provide better evidence but they are either prevented from doing so or failed to do so). I am inclined to believe special interests have more sway in agencies like (NASA, EPA, FDA…) than the public good and scientific openness – which is very sad. And, it seems to me, politicians have overwhelmingly chosen not to support more science in places like FDA, CDC, NIH… while increasing federal spending in other areas dramatically.

Related: USDA’s failure to protect the food supplyFDA May Make Decision That Will Speed Antibiotic Drug ResistanceFood safety proposal: throw the bums outThe A to Z Guide to Political Interference in Science

Exploring the Signaling Pathways of Cells

New probe may help untangle cells’ signaling pathways

MIT researchers have designed a new type of probe that can image thousands of interactions between proteins inside a living cell, giving them a tool to untangle the web of signaling pathways that control most of a cell’s activities.

“We can use this to identify new protein partners or to characterize existing interactions. We can identify what signaling pathway the proteins are involved in and during which phase of the cell cycle the interaction occurs,” said Alice Ting, the Pfizer-Laubach Career Development Assistant Professor of Chemistry and senior author of a paper describing the probe published online June 27 by the Journal of the American Chemical Society.

The new technique allows researchers to tag proteins with probes that link together like puzzle pieces if the proteins interact inside a cell. The probes are derived from an enzyme and its peptide substrate. If the probe-linked proteins interact, the enzyme and substrate also interact, which can be easily detected.

To create the probes, the researchers used the enzyme biotin ligase and its target, a 12-amino-acid peptide.

Related: Specific Protein and RNA Labeling in CellsUsing Bacteria to Carry Nanoparticles Into CellsMolecular Bioengineering and Dynamical Models of CellsThe Inner Life of a Cell (Animation)

S&P 500 CEOs are Engineering Graduates

2007 Data from Spencer Stuart on S&P 500 CEO (they deleted the link so the link was removed – yet another website proves to be unreliable without basic web usability principles being followed) shows once again more have undergraduate degrees in engineering than any other field.

Field
   
% of CEOs
2007 2006 2005

Engineering 21 23 20
Economics 15 13 11
Business Administration 13 12 15
Accounting 8 8 7
Liberal Arts 6 8 9
No degree or no data 3 3

The report does not show the fields for the rest of the CEO’s. 40% of S&P CEOs have MBAs. 27% have other advanced degrees. The University of Wisconsin-Madison, Princeton and Harvard tied for the most CEO’s with undergraduate degrees from their universities at 12. University of Texas has 10 and Stanford has 9.

Data for previous years is also from Spencer Stuart: 2006 S&P 500 CEO Education StudyTop degree for S&P 500 CEOs? Engineering (2005 study)

Related: Engineering Education Study Debateposts on science and engineering careersScience and Engineering Degrees lead to Career SuccessThe Future is Engineering

Pesticide Laced Fertiliser Ruins Gardens

Home-grown veg ruined by toxic fertiliser

Aminopyralid, which is found in several Dow products, the most popular being Forefront, a herbicide, is not licensed to be used on food crops and carries a label warning farmers using it not to sell manure that might contain residue to gardeners. The Pesticides Safety Directorate, which has issued a regulatory update on the weedkiller, is taking samples from affected plants for testing.

Problems with the herbicide emerged late last year, when some commercial potato growers reported damaged crops. In response, Dow launched a campaign within the agriculture industry to ensure that farmers were aware of how the products should be used. Nevertheless, the herbicide has now entered the food chain. Those affected are demanding an investigation and a ban on the product. They say they have been given no definitive answer as to whether other produce on their gardens and allotments is safe to eat.

It appears that the contamination came from grass treated 12 months ago. Experts say the grass was probably made into silage, then fed to cattle during the winter months. The herbicide remained present in the silage, passed through the animal and into manure that was later sold. Horses fed on hay that had been treated could also be a channel.

Related: Effect of People on Other SpeciesPigs Instead of PesticidesPeak SoilFlushed Drugs Pollute Water

Cell Signals Webcast

Very cool animation, by Cold Spring Harbor Laboratory and Interactive Knowledge, of the working of the inner workings of our bodies as they react to a cut. If you want to get right to the science, skip the first minute. Providing these types of educational animations is a great way for educational institutions to take advantage of technology to achieve their mission in ways not possible before.

It is annoying how many of those “educational” institutions don’t provide such educational material online (and even take material offline that was online). Have they become more focused on thinking and operating the way they did in 1970 than promoting science education? It is a shame some “educational” institutions have instead become focused on looking backward. I will try to promote those organizations that are providing online science education.

Related: Inside Live Red Blood CellsUniversal Blood

2008 Lemelson-MIT Prize for Invention

photo of Joseph Desimone

The Lemelson-MIT Prize awards $500,000 to mid-career inventors dedicated to improving our world through technological invention and innovation. Joseph M. DeSimone received the 2008 award.

His exposure to polymer science led him to pursue a Ph.D. in chemistry from Virginia Polytechnic Institute and State University in Blacksburg, Va. At the age of 25, DeSimone joined the University of North Carolina at Chapel Hill (UNC) as an assistant professor in chemistry and launched the university’s polymer program with his mentor Dr. Edward Samulski. He resides there today as the Chancellor’s Eminent Professor of Chemistry at UNC, in addition to serving as the William R. Kenan, Jr. Distinguished Professor of Chemical Engineering at North Carolina State University.

Among DeSimone’s notable inventions is an environmentally friendly manufacturing process that relies on supercritical carbon dioxide instead of water and bio-persistent surfactants (detergents) for the creation of fluoropolymers or high-performance plastics, such as Teflon®. More recently, he worked on a team to design a polymer-based, fully bioabsorbable, drug-eluting stent, which helps keep a blocked blood vessel open after a balloon-angioplasty and is absorbed by the body within 18 months.

DeSimone’s newest invention is PRINT® (Particle Replication in Non-wetting Templates) technology, used to manufacture nanocarriers in medicine. At present, DeSimone’s Lab is vested in a variety of projects that also extend beyond medicine, including potential applications for more efficient solar cells and morphable robots. In 2004, DeSimone co-founded Liquidia Technologies with a team of researchers from UNC to make the technology available in the market. Liquidia is using the PRINT technology to develop precisely engineered nanocarriers for highly targeted delivery of biological and small molecule therapeutics to treat cancer and other diseases. DeSimone’s proposed applications for cancer treatment with the PRINT platform was instrumental in UNC landing a grant of $24 million from the National Cancer Institute to establish the Carolina Center for Cancer Nanotechnology Excellence.

“You can do all the innovating you want in the laboratory, but if you can’t get it out of the university walls you do no one any good,” said DeSimone. He instills an entrepreneurial spirit in his students that focuses on the importance of commercializing technology and scientific inventions. One of DeSimone’s greatest accomplishments is his mentorship of more than 45 postdoctoral research associates, 52 Ph.D. candidates, six M.S. theses and 21 undergraduate researchers. Furthermore, he speaks to groups of high school students about the inventive process and encourages them to learn and explore areas that are less familiar to them to broaden their exposure to other disciplines.

A prolific inventor, DeSimone holds more than 115 issued patents with more than 70 new patent applications pending, and he has published more than 240 peer-reviewed scientific articles.

Related: Inspiring a New Generation of Inventors$500,000 for Innovation in Engineering EducationCollegiate Inventors Competitionposts on inventors

Our Genome Changes as We Age

Our Genome Changes Over Our Lifetime

For the new study, researchers first collected DNA samples collected in 1991 and again between 2002 and 2006 from 600 participants already enrolled in the AGES Reykjavik Study. The AGES study is renowned for its value to genetics research because of the historic isolation and reduced number of genetic “variables” among Iceland’s population, making certain patterns of genetic information easier to identify.

Among the 600, the research team measured the total amount of DNA methylation in each of 111 samples and compared total methylation from DNA collected in 2002 to 2005 to that person’s DNA collected in 1991.

They discovered that in almost one-third of the subjects, methylation changed over that 11-year span, with some gaining DNA methylation and others losing it.

“The key thing this part of the study told us is that levels changed over time, proof of principle that an individual’s epigenetic profile does change with age,” said M. Daniele Fallin, Ph.D., an associate professor of epidemiology at the Johns Hopkins Bloomberg School of Public Health.

Still a puzzle, though, was why or how, Fallin said, “so we wondered whether the tendency to those changes was also inherited, right along with our DNA sequences. That would explain why certain families are more susceptible to certain diseases.”

Related: Genetic Information Nondiscrimination ActLearning About the Human GenomeGenomics Course For College Freshman Supported by HHMI at 12 Universities

Transferring Train Passengers Without Stopping

The webcast shows a train transferring passengers without stopping. Essentially passenger modules are picked up and dropped off at each station. Looks pretty cool and would seem to require somewhat complex engineering – which can be a problem as complexity allows for more things to go wrong. Still it looks pretty cool. The sound is not in English but you can see what the idea is.

Inventor rolls out efficient non-stop train system

Taking the Kaohsiung MRT system as an example, Peng says that its maximum speed is 85 kph. Because it must stop at every station, it achieves an average speed over its route of just 35 kph. If the non-stop system were in place, the top velocity of 85 kph could be maintained throughout the system, saving time and energy.

via: trains that pick you up without stopping

Related: Extreme EngineeringMIT Hosts Student Vehicle Design SummitDesigning Cities for People, Rather than Cars