Tag Archives: open access paper

Genes Counter a Bacterial Attack

Gene against bacterial attack unravelled

Humans have an innate defence system against deadly bacteria. However, how the step from gene to anti-bacterial effect occurs in the body is not yet known. To date, B. Pseudomallei, a bacterium suitable for bioweapons, had managed to elude medics. It can remain hidden in the human body for many years without being detected by the immune system. The bacteria can suddenly become activated and spread throughout the body, resulting in the patient dying from blood poisoning. AMC physician Joost Wiersinga and the Laboratory for Experimental Internal Medicine discovered which gene-protein combination renders the lethal bacteria B. pseudomallei harmless.

Wiersinga focussed on the so-called Toll-like receptors. These are the proteins that initiate the fight against pathogens. There are currently ten known Toll-like receptors which are located on the outside of immune cells, our body’s defence system. The toll-like receptors jointly function as a 10-figure alarm code. Upon coming into contact with the immune cell each bacterium enters its own Toll code. For known pathogens this sets off an alarm in the immune system and the defence mechanism is activated. Yet B. pseudomallei fools the system by entering the code of a harmless bacterium. As a result the body’s defence system remains on standby.

Yet some people are resistant: they become infected but not ill. Wiersinga found a genetic cause for this resistance. He discovered which toll receptor can fend off B. pseudomallei. He did this by rearing mice DNA in which the gene for Toll2 production was switched on and off. ‘The group where the gene for Toll2 was switched off, survived the bacterial infection’, says Wiersinga. ‘The other receptor that we investigated, Toll4, had no effect – even though for the past ten years medics had regarded this as the most important receptor.’ The ultimate aim of this study is to develop a vaccine.

PLoS paper: MyD88 Dependent Signaling Contributes to Protective Host Defense against Burkholderia pseudomallei

Waste from Gut Bacteria Helps Host Control Weight

A single molecule in the intestinal wall, activated by the waste products from gut bacteria, plays a large role in controlling whether the host animals are lean or fatty, a research team, including scientists from UT Southwestern Medical Center, has found in a mouse study.

When activated, the molecule slows the movement of food through the intestine, allowing the animal to absorb more nutrients and thus gain weight. Without this signal, the animals weigh less.

The study shows that the host can use bacterial byproducts not only as a source of nutrients, but also as chemical signals to regulate body functions. It also points the way to a potential method of controlling weight, the researchers said.

“It’s quite possible that blocking this receptor molecule in the intestine might fight a certain kind of obesity by blocking absorption of energy from the gut,” said Dr. Masashi Yanagisawa, professor of molecular genetics at UT Southwestern and a senior co-author of the study, Proceedings of the National Academy of Sciences, open access: Effects of the gut microbiota on host adiposity are modulated by the short-chain fatty-acid binding G protein-coupled receptor, Gpr41.

Humans, like other animals, have a large and varied population of beneficial bacteria that live in the intestines. The bacteria break up large molecules that the host cannot digest. The host in turn absorbs many of the resulting small molecules for energy and nutrients.

In the Big Fat Lie I mentioned some related ideas:

It also makes perfect sense that our bodies evolved to store energy for worse times (and some of us have bodies better at doing that). Now we are in a new environment where (at least for many people alive today) finding enough calories is not going to be a problem so it would be nice if we could tell our bodies to get less efficient at storing fat

This research seems to be looking for a similar way to attack the obesity epidemic: reduce the efficiency of our bodies converting potential energy in the food we eat to energy we use or store. If we can make that part of the solution that will be nice. So far the reduction in our activity and increase in food intake have not been getting good results. And efforts to increase (from our current low levels) activity and reduce food intake have not been very effective.
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Algorithmic Self-Assembly

Paul Rothemund, scientist at Cal Tech, provides a interesting look at DNA folding and DNA based algorithmic self-assembly. In the talk he shows the promise ahead for using biological building blocks using DNA origami — to create tiny machines that assemble themselves from a set of instructions.

Algorithmic Self-Assembly of DNA Sierpinski Triangles, PLoS paper.

I posted a few months ago about how you can participate in the protein folding, with the Protein Folding Game.

Speciation of Dendroica Warblers

Speciation for Dendroica Warblers

They developed a mathematical model that attributed patterns of speciation to the way that closely related species divide up their environment. According to this model, when there are few relatives around to compete for resources, such as when an environment is first colonized, species differentiate rapidly.
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This model is robust: even when the authors assumed that their phylogenetic tree contains only 25 percent of all Dendroica species, they found that their Î³ test was still valid, indicating that this genus experienced an explosive bout of adaptive radiation before settling down to a “more normal” rate of speciation.

This mathematical model provides an incisive tool to gain a clearer understanding of the pattern and rate of speciation for groups of closely related species, even in the absence of a fossil record, simply by analyzing their DNA.

Materials Engineers Create Perfect Light “sponge”

Materials engineers create perfect light “sponge”

The team designed and engineered a metamaterial that uses tiny geometric surface features to successfully capture the electric and magnetic properties of a microwave to the point of total absorption.

“Three things can happen to light when it hits a material,” says Boston College Physicist Willie J. Padilla. “It can be reflected, as in a mirror. It can be transmitted, as with window glass. Or it can be absorbed and turned into heat. This metamaterial has been engineered to ensure that all light is neither reflected nor transmitted, but is turned completely into heat and absorbed. It shows we can design a metamaterial so that at a specific frequency it can absorb all of the photons that fall onto its surface.”
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The metamaterial is the first to demonstrate perfect absorption and unlike conventional absorbers it is constructed solely out of metallic elements, giving the material greater flexibility for applications related to the collection and detection of light, such as imaging, says Padilla, an assistant professor of physics.

Bird Brain Language Research

Molecular Mapping of Movement-Associated Areas in the Avian Brain: A Motor Theory for Vocal Learning Origin

Vocal learning is a critical behavioral substrate for spoken human language. It is a rare trait found in three distantly related groups of birds-songbirds, hummingbirds, and parrots. These avian groups have remarkably similar systems of cerebral vocal nuclei for the control of learned vocalizations that are not found in their more closely related vocal non-learning relatives. These findings led to the hypothesis that brain pathways for vocal learning in different groups evolved independently from a common ancestor but under pre-existing constraints. Here, we suggest one constraint, a pre-existing system for movement control.

Using behavioral molecular mapping, we discovered that in songbirds, parrots, and hummingbirds, all cerebral vocal learning nuclei are adjacent to discrete brain areas active during limb and body movements. Similar to the relationships between vocal nuclei activation and singing, activation in the adjacent areas correlated with the amount of movement performed and was independent of auditory and visual input.
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Based upon these findings, we propose a motor theory for the origin of vocal learning, this being that the brain areas specialized for vocal learning in vocal learners evolved as a specialization of a pre-existing motor pathway that controls movement.

Laws of Physics May Need a Revision

Something seems wrong with the laws of physics

Einstein’s general theory of relativity swept Newton away by showing that gravity operates by distorting space itself.

Even Einstein, however, may not have got it right. Modern instruments have shown a departure from his predictions, too. In 1990 mission controllers at the Jet Propulsion Laboratory (JPL) in Pasadena, California, which operates America’s unmanned interplanetary space probes, noticed something odd happen to a Jupiter-bound craft, called Galileo. As it was flung around the Earth in what is known as a slingshot manoeuvre (designed to speed it on its way to the outer solar system), Galileo picked up more velocity than expected. Not much. Four millimetres a second, to be precise. But well within the range that can reliably be detected.
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Altogether, John Anderson and his colleagues analysed six slingshots involving five different spacecraft. Their paper on the matter is about to be published in Physical Review Letters. Crucially for the idea that there really is a systematic flaw in the laws of physics as they are understood today, their data can be described by a simple formula. It is therefore possible to predict what should happen on future occasions.

That is what Dr Anderson and his team have now done. They have worked out the exact amount of extra speed that should be observed when they analyse the data from a slingshot last November, which involved a craft called Rosetta. If their prediction is correct, it will confirm that the phenomenon is real and that their formula is capturing its essence. Although the cause would remain unknown, a likely explanation is that something in the laws of gravity needs radical revision.

An interesting puzzle that illustrates how scientists attempt to confirm our understanding and real world results. And those efforts include uncertainty and confusion. Too often, I think, people think science is only about absolute truth and facts without any room for questions. We understand gravity well, but that does not mean we have no mysteries yet to solve about gravity.

Research paper: The Anomalous Trajectories of the Pioneer Spacecraft

Antarctic Fish “Hibernate” in Winter

This is the first time fish have been seen actively becoming torpid—a state similar to hibernation in land animals—as part of an annual cycle. “A lot of freshwater fish go [unexpectedly] dormant in winter because a drop in temperature lowers their metabolism,” said study co-author Hamish Campbell, a zoologist at the University of Queensland, Australia.

“By contrast, these Antarctic fish actively reduce their ‘cost of living,'” he said… “The fish became 20 times less active in winter compared to summer,”… About every week or so the cod wake up and swim around for a few hours, the team observed. “This is quite similar to ‘denning’ in bears, where the hibernation isn’t so deep and the animals can be disturbed, then spend some time awake before going back to bed,” Fraser said.

Full paper: Hibernation in an Antarctic Fish: On Ice for Winter – Arctic Sharks – Antarctic Robo-sub

Placebo Effect

Don’t laugh, sugar pills are the future

In fact the new study added nothing (and it was ridiculously badly reported): we already knew that antidepressants perform only marginally better than placebo, and the National Institute for Health and Clinical Excellence (Nice) guidelines has actively advised against using them in milder depression since 2004. But the more interesting questions are around placebo.
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Another study from 2002 looked at 75 trials of antidepressants over the past 20 years, but looked only at the response in the placebo arms of the trials, and found that the response to placebo has increased significantly in recent years (as has the response to medication): perhaps our expectations of those drugs have increased, or perhaps, conversely to our earlier example, the trial designs have become systematically more flattering. I’m giving you tenuous data, on an interesting area, because I know you’re adult enough to cope with ambiguity.

Electron Filmed for the First Time

Now it is possible to see a movie of an electron. The movie shows how an electron rides on a light wave after just having been pulled away from an atom. This is the first time an electron has ever been filmed. Previously it has been impossible to photograph electrons since their extremely high velocities have produced blurry pictures. In order to capture these rapid events, extremely short flashes of light are necessary, but such flashes were not previously available.

With the use of a newly developed technology for generating short pulses from intense laser light, so-called attosecond pulses, scientists at the Lund University Faculty of Engineering in Sweden have managed to capture the electron motion for the first time. “It takes about 150 attoseconds for an electron to circle the nucleus of an atom. An attosecond is 10-18 seconds long, or, expressed in another way: an attosecond is related to a second as a second is related to the age of the universe,” says Johan Mauritsson, an assistant professor in atomic physics at the Faculty of Engineering, Lund University.

Scientists also hope to find out more about what happens with the rest of the atom when an inner electron leaves it, for instance how and when the other electrons fill in the gap that is created. “What we are doing is pure basic research. If there happen to be future applications, they will have to be seen as a bonus,” adds Johan Mauritsson. The length of the film corresponds to a single oscillation of the light, but the speed has then been ratcheted down considerably so that we can watch it. The filmed sequence shows the energy distribution of the electron and is therefore not a film in the usual sense.

Photo: Experimental results obtained in helium at an intensity of 1:2 x 1013 W=c/m2 are shown. The results are
distinctively different from those taken in argon (Fig. 1).With this higher intensity, more momentum is transferred to the electrons, and in combination with the lower initial energy, some electrons return to the atomic potential for further interaction. In the first panel, we compare the experimental results (right) with theoretical calculations (left) obtained for the same conditions.
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